By Rodolfo Paoletti, Dr. David Kritchevsky
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Additional info for Advances in lipid research. Volume 6
For the preparation of antisera of high antibody titer it is advisable to "boost" the animal every 3 — 5 weeks exactly as for the first injection. Good antiserum is usually obtained after two injections.
A quantity of lipoprotein containing 5 — 20 mg of protein, as measured by the method of Lowry et al. (1951) in about 1 ml of saline or barbital buffer is emulsified with an equal volume of Freund's complete adjuvant (Difco Laboratories, Detroit, Michigan). This is most easily done by placing the lipoprotein and the adjuvant in separate 5ml Luer-Lock syringes with 20-gauge needles and connecting the two needles via a 1-in. 030 in. d. polyethylene tubing. 21 ß sf sf Sf 0-400 /3 + Pre-/3 "Three populations — 77 cases.
The constant voltage mode is recommended, and the direc- Plasma Lipoprotein Analysis 37 tions will be so given. Successful results may also be obtained with the constant current mode, but a higher starting voltage must be determined by experiment to compensate for the decrease in voltage which occurs during the run. FlG. 3. Staining tank for paper and agarose electrophoretic strips. Optimal staining is carried out by hanging the strips vertically by means of chromatography clips (stainless steel or plastic) from wires traversing a battery jar (Catalog No.
Advances in lipid research. Volume 6 by Rodolfo Paoletti, Dr. David Kritchevsky